| | Category | BO | L10 | Antibacterial activity of S.African plants against P. intermedia & A. |
| | israelli |
| | Abstract | Abstract: |
| | The purpose of this project was to determine whether or not the selected |
| | plants in the study could inhibit the growth of the periodontal bacteria |
| | Actinomyces israelli and Prevotella intermedia. The plants tested were |
| | Euclea natalensis (leaves and roots), Leucosidea sericea (leaves), |
| | Pelargonium luridum (leaves), Pelargonium reniforme (leaves and roots), |
| | and Sanseviera hyacinthoides (leaves). This was tested by preparing |
| | extracts of the selected plants and placing them (in different |
| | concentrations) in the individual wells of microtitre plates containing the |
| | two bacteria and a control. The bacteria were grown in Tryptone Soy |
| | Agar and kept alive in the plates by feeding on Tryptone Soy Broth. The |
| | plates were sealed and incubated under anaerobic conditions for 48 hours |
| | at 37 °C. P-iodonitrotetrazolium (INT) was used as the detection reagent |
| | for the Minimum Inhibitory Concentration. This was determined where no |
| | pink occurred in the wells. After another 48 hours, INT was added to the |
| | untouched wells to determine the Minimum Bactericidal Concentration. The |
| | color intensity was measured using an Elisa plate reader to get the dose |
| | response curve. All but two plants showed activity against the two |
| | bacteria. The plant Leucosidea sericea provided the best results by killing |
| | most of the bacteria. The concentration of the bacteria in the wells that |
| | tested the activity of this plant was ≤0.097656 for the MIC and |
| | 3.125 for the MBC. |
| | Bibliography | http://www.medicineword.com/Actinomyces+israelii.shtmlhttp://books.goo |
| | gle.com/books?id=YVwMM2OdO34C&pg=PA28&lpg=PA28&dq=%22Sanse |
| | vieria+hyacinthoides%22+medicinal+use&source=web&ots=xfSAawHdkW |
| | &sig=6tLn5Kyz0mGSvaatDtZAuPPw2Es&hl=en&sa=X&oi=book_result&res |
| | num=4&ct=result#PPA29,M1 |